Journal: Journal of Functional Biomaterials

Article Title: Prosthetic Joint Infection Research Models in NZW Rabbits: Opportunities for Standardization—A Systematic Review

doi: 10.3390/jfb15100307

Figure Lengend Snippet: Experimental design extraction results—part 1. Abbreviations used: ns = not stated.

Article Snippet: [ ] , To study the effectiveness of gentamicin-loaded PNDJ (G-PNDJ) hydrogels in treating orthopaedic infections in a rabbit model. , 49 , S. aureus UAMS-1, ATCC 4923 , 7.5 × 10 6 CFU in 50 μL TSB , Inoculated after wire implantation, unspecified how , None.

Techniques: Extraction, Animal Model, Infection, Saline, Injection, Imaging, Activation Assay, Control, In Vivo, Molecular Weight, Modification, In Vitro, Comparison, Activity Assay, Incubation, Transferring, Polymer, Encapsulation, Bacteria, Isolation, Sterility, Bioprocessing

Journal: Advanced Science

Article Title: Western Diet Inhibits FUT7‐Mediated Treg Intestinal Homing to Disrupt the Homeostasis of Intestinal Epithelial Cells in Crohn's Disease

doi: 10.1002/advs.202509541

Figure Lengend Snippet: Upregulation of Fut7 in Tregs improves WD‐induced intestinal barrier disruption in mice. A – L ) Littermate WT mice were divided into four groups ( n = 8/group). They were fed with a normal diet (RD) in group 1 and a western diet (WD) in groups 2–4 for 17 weeks. In week 17, the positive nanoparticle (PN) CD4‐LDP‐Fut7 and the negative nanoparticle (NN) CD4‐LDP‐NC were given by tail intravenous injection at a dose of 240 ng/g once every two days for a total of three times in groups 3 and 4, respectively. ( A ) Fut7 mRNA expression in FACS‐sorted Tregs from the spleen. ( B ) Representative flow cytometric plots of colonic CD25 + Foxp3 + Tregs. ( C ) The frequency of CD25 + Foxp3 + Tregs in CD4 + T cells. ( D ) Colon length. ( E ) Representative gross morphology of the colon. ( F ) After 4 h of fasting, the mice were given FITC‐conjugated dextran by gavage administration for 4 h, and subsequently, the concentration of FITC‐dextran in blood serum was measured. ( G ) Representative intestinal H&E staining. ( H ) Mucosal injury index. ( I ) Inflammatory cell infiltration index. ( J ) Representative immunofluorescence images of Occludin and ZO‐1 immunostaining in colonic tissues. ( K, L ) MFI of Occludin and ZO‐1 was quantified by ImageJ software. The data shown are representative of three independent experiments. ( A, C, D, F, K, L ) one‐way ANOVA or ( H, I ) Kruskal‐Wallis test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant. FACS, fluorescence‐activated cell sorting; MFI, mean fluorescence intensity; WT, wild‐type.

Article Snippet: A naive CD4 + T cell adoptive transfer model was established using Rag2 −/− mice purchased from Cyagen Biosciences (Guangzhou, China) according to a published procedure.

Techniques: Disruption, Western Blot, Injection, Expressing, Concentration Assay, Staining, Immunofluorescence, Immunostaining, Software, Fluorescence, FACS

Journal: Advanced Science

Article Title: Western Diet Inhibits FUT7‐Mediated Treg Intestinal Homing to Disrupt the Homeostasis of Intestinal Epithelial Cells in Crohn's Disease

doi: 10.1002/advs.202509541

Figure Lengend Snippet: Specific deletion of Fut7 in Tregs deteriorates TNBS‐induced intestinal barrier disruption in mice. A ) fut7 fl/fl foxp3 cre mice with conditional knockout (CKO) of the fut7 gene in Tregs were generated as shown in the schematic diagram. B ) The knockout efficiency was determined by Western blot analysis of Fut7 expression in FACS‐sorted splenic Tregs. C ) Representative flow cytometric plots of colonic CD25 + Foxp3 + Tregs. D ) The frequency of CD25 + Foxp3 + Tregs in CD4 + T cells. E–K ) CKO and fut7 fl/fl littermates were intrarectally injected with TNBS to induce colitis (n = 6–8/group). ( E ) Mouse weight changes. ( F ) Colon length. ( G ) Representative gross morphology of the colon. ( H ) After 4 h of fasting, the mice were given FITC‐conjugated dextran by gavage administration for 4 h, and subsequently, the concentration of FITC‐dextran in blood serum was measured. ( I ) Representative intestinal H&E staining. ( J ) Mucosal injury index. ( K ) Inflammatory cell infiltration index. Data shown in C–K are representative of three independent experiments. ( D ) unpaired Student's t test, ( E , F, H ) one‐way ANOVA, or ( J, K ) Kruskal‐Wallis test; * P < 0.05, ** P < 0.01, **** P < 0.0001; ns, not significant. FACS, fluorescence‐activated cell sorting; TNBS, 2,4,6‐trinitrobenzenesulfonic acid.

Article Snippet: A naive CD4 + T cell adoptive transfer model was established using Rag2 −/− mice purchased from Cyagen Biosciences (Guangzhou, China) according to a published procedure.

Techniques: Disruption, Knock-Out, Generated, Western Blot, Expressing, Injection, Concentration Assay, Staining, Fluorescence, FACS

Journal: Advanced Science

Article Title: Western Diet Inhibits FUT7‐Mediated Treg Intestinal Homing to Disrupt the Homeostasis of Intestinal Epithelial Cells in Crohn's Disease

doi: 10.1002/advs.202509541

Figure Lengend Snippet: FUT7 expression in Tregs and CD15s + Tregs is decreased in patients with active CD. A ) Fut7 mRNA expression in FACS‐sorted Tregs from the blood of patients with active CD (n = 6), quiescent CD (n = 6), and healthy controls (n = 6). B–E ) PBMCs were isolated from the blood of patients with active CD (n = 8), quiescent CD (n = 8), and healthy controls (n = 8) by density centrifugation, and then CD4 + CD25 + CD127 low Tregs and CD15s + Tregs were analyzed by flow cytometry. ( B, D ) Representative flow cytometric plots. ( C ) The frequency of CD25 + CD127 low Tregs in CD4 + T cells. ( E ) The frequency of CD15s + Tregs in Treg cells. ( F to H ) Fresh colonic mucosa from active CD ( n = 9), quiescent CD ( n = 9), and healthy controls ( n = 9) were fixed in 4% paraformaldehyde and subjected to immunofluorescence staining using anti‐human antibodies targeting Foxp3 and CD15s. ( F ) Representative immunofluorescence images. ( G, H ) The number of indicated Treg subsets. ( A, C, E, G, H ) One‐way ANOVA; ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant. FACS, fluorescence‐activated cell sorting; PBMC, peripheral blood mononuclear cell.

Article Snippet: A naive CD4 + T cell adoptive transfer model was established using Rag2 −/− mice purchased from Cyagen Biosciences (Guangzhou, China) according to a published procedure.

Techniques: Expressing, Isolation, Centrifugation, Flow Cytometry, Immunofluorescence, Staining, Fluorescence, FACS

Journal: Physical Chemistry Chemical Physics

Article Title: The SAMPL9 host–guest blind challenge: an overview of binding free energy predictive accuracy †

doi: 10.1039/d3cp05111k

Figure Lengend Snippet: Summary of methods (ranked and non-ranked) used in the SAMPL9 host–guest challenge for binding free energy calculations. Explicit and/or implicit solvents are flagged by an (E) or (I) respectively. If a correction approach was taken by a method, it is flagged with a (C). If the host (H) and guest (G) are parameterized with a different energy model, they will be flagged respectively. If a machine learning or docking approach was taken, certain categories are not relevant and flagged with an asterisk (*). Reference calculations are flagged with a double asterisk (**)

Article Snippet: Using an ML model with molecular descriptors or an empirical approach from AutoDock Vina's smina scoring function for host–guest systems has a speed and perhaps cost effective advantage for BFE accuracy.

Techniques: Binding Assay, Solvent, Sampling

Journal: Physical Chemistry Chemical Physics

Article Title: The SAMPL9 host–guest blind challenge: an overview of binding free energy predictive accuracy †

doi: 10.1039/d3cp05111k

Figure Lengend Snippet: Computed error metrics of all methods with submissions for SAMPL9 host–guest systems. The error metrics computed include the root mean square error (RMSE), mean absolute error (MAE), signed mean error (ME), coefficient of correlation ( R 2 ), slope ( m ), and Kendall's rank correlation coefficient (Tau). The results shown are for each host category and are sorted in an ascending order based on RMSE. The metrics were computed via bootstrapping with replacement and the upper and lower bounds of 95%-percentile confidence intervals are shown in brackets. The statistics in this table do not include the optional host–guest system WP6-G4. Each unique method has an assigned submission ID (sid), a ranked submission is denoted with an asterisk next to the method name, and a reference submission is flagged with a double asterisk

Article Snippet: Using an ML model with molecular descriptors or an empirical approach from AutoDock Vina's smina scoring function for host–guest systems has a speed and perhaps cost effective advantage for BFE accuracy.

Techniques:

Journal: Journal of Functional Biomaterials

Article Title: Prosthetic Joint Infection Research Models in NZW Rabbits: Opportunities for Standardization—A Systematic Review

doi: 10.3390/jfb15100307

Figure Lengend Snippet: Experimental design extraction results—part 1. Abbreviations used: ns = not stated.

Article Snippet: [ ] , In this study, the post porous hydroxyapatite (HA) coated Ti4Al4V is prepared for the subsequent electrolytic deposition of vancomycin–chitosan composite to control the drug release. The aim of this study is to test the antibacterial effect in a rabbit infection animal model. , 28 , S. aureus ATCC 6538P , 10 7 CFU/20 μL , Injected with 16G needle before insertion pin , None.

Techniques: Extraction, Animal Model, Infection, Saline, Injection, Imaging, Activation Assay, Control, In Vivo, Molecular Weight, Modification, In Vitro, Comparison, Activity Assay, Incubation, Transferring, Polymer, Encapsulation, Bacteria, Isolation, Sterility, Bioprocessing